We have four principal services.
- We develop novel dye or antibody-based cytometry assays.
- We optimize and bring existing protocols to a standard of practice.
- We receive samples, process, and perform cytometry.
- We analyze data and create custom reports.
We develop assays for flow cytometry. Typically, the assays include antibodies to define cell type; antibodies that are target epitopes; and dyes (live/dead, cell cycle). A typical development cycle includes:
- Selection of antibodies.
- Testing antibodies for binding and specificity.
- Selection of positive and negative cell controls.
- Identification of appropriate bead-based performance and calibration.
- Optimization of fixation and permeabilization.
- Optimization of staining.
- Sample handling and shipping evaluations.
- Small test with typical samples.
- Write SOP.
We optimize and reduce to an SOP assays from vendors, laboratories, and/or the literature.
- Obtain current copy of existing protocol.
- Identify uncertain or conflicting instructions.
- Determine the fit of existing protocol to current study.
- Resolve uncertainties.
- Identify and obtain reagents.
- Determine sample format (e.g., fresh, cryo-preserveed, fixed).
- Formulate end points and performance criteria.
- Perform provisional protocol.
- Modify protocol as necessary.
- Identify and test performance and calibrator beads and cellular controls.
- Perform updated provisional protocol.
- Write up and log SOP.
We will process samples for studies using new or existing protocols that we have reduced to an SOP. The steps involved are:
- Develop or adopt an assay.
- Process the samples. Batch mode preferred.
- Perform measurements and analyze data.
Available Instruments:
Flow cytometry: Thermo-Fisher Attune (4 lasers, 13 colors).
We deliver raw data to a secure location.
We analyze data according to customer approved protocol.
We develop and validate analytical protocols.
We analyze data by internally developed protocols.
We write and deliver custom reports and graphics.
Software: Manufacturers instrument specific software, Verity Software House (WinList, ModFit LT)